Peptide Reconstitution Calculator
Enter your vial details and desired dose below. The calculator will show you exactly how much to draw on your syringe — with a visual guide.
Results
1.0 mL (U-100) Syringe
Draw to the 5.0 unit mark (0.050 mL)
Reconstitution is the process of mixing a lyophilized (freeze-dried) peptide powder with a sterile liquid — typically bacteriostatic water (BAC water) — to create an injectable solution.
Most peptides are shipped as a delicate powder in a sealed vial. Before you can accurately measure and inject a dose, you must dissolve the powder by adding a precise amount of BAC water. The amount of water you add determines the concentration of the resulting solution, which directly affects how much liquid you need to draw for each dose.
- Clean both vial stoppers with an alcohol swab — the BAC water vial and the peptide vial.
- Draw the desired volume of BAC water using a fresh syringe. Use the amount entered above in the calculator.
- Inject slowly into the peptide vial, aiming the stream against the glass wall — not directly onto the powder.
- Swirl gently until the powder is fully dissolved. Never shake the vial, as this can denature the peptide and reduce efficacy.
Once reconstituted, peptides become much more fragile than in their lyophilized form. Proper storage is essential for maintaining potency:
- Refrigerate immediately at 36-46 degrees F (2-8 degrees C). Standard refrigerator temperature is ideal.
- Protect from light. Keep the vial in its box or wrap it in foil.
- Use within 28-30 days of reconstitution for most peptides. The preservative in BAC water (benzyl alcohol 0.9%) helps prevent microbial growth but has a limited effective window.
- Never freeze a reconstituted peptide solution — ice crystals will destroy the peptide structure.
This tool is for educational purposes only. It is intended to help you understand how reconstitution math works and to verify calculations provided by your healthcare provider.
Always follow your provider's instructions for reconstitution volumes, dosing amounts, injection frequency, and storage requirements. Do not self-prescribe or adjust dosages without medical guidance.
PrimeHealth provides physician-supervised peptide therapy with personalized dosing protocols. If you have questions about your treatment, contact your assigned provider directly.